Journal of Cytokine Biology: Intracellular Staining.


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Journal of Cytokine Biology: Intracellular Staining.

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Intracellular cytokine staining

Intracellular protein staining (ICS) could be a terribly helpful and wide used flow cytometry primarily based assay that detects the assembly and accumulation of cytokines at intervals the endoplasmic reticulum once cell stimulation. ICS may be utilized in combination with different flow cytometry protocols for immunopheno typing mistreatment cell surface markers or with MHC multimers to observe Associate in Nursing substance specific response, creating it a very versatile and versatile technique. Cells square measure activated mistreatment either a particular amide or a non-specific activation cocktail. Associate in Nursing substance of macromolecule transport is accessorial to retain the cytokines at intervals the cell. once laundry, antibodies to different cellular markers may be accessorial to the cells. The cells square measure then fastened in paraformaldehyde and permeabilized. The anti-cytokine protein is accessorial and also the cells may be analyzed by flow cytometer.


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Veronica Thompson

Journal Manager
Journal of Cytokine Biology